Mitotically Synchronized Mammalian Cells: A Simple Method for Obtaining Large Populations

Science  29 May 1964:
Vol. 144, Issue 3622, pp. 1152-1153
DOI: 10.1126/science.144.3622.1152


Eliminating most of the ionic calcium from growth medium does not affect cell growth, but it allows simple and preferential detachment of HeLa cells in mitosis from nearly confluent monolayers.