Nucleotides: Separation from an Alkaline Hydrolysate of RNA by Thin-Layer Electrophoresis

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Science  12 Jun 1964:
Vol. 144, Issue 3624, pp. 1350-1351
DOI: 10.1126/science.144.3624.1350


Mixtures of the 2', 3' nucleoside monophosphates obtained from an alkaline hydrolysis of RNA can be resolved into the four main RNA mononucleotides by electrophoresis on thin layers of cellulose. The separated nucleotides are recovered by removing the cellulose layer from the supporting glass plate and suspending the cellulose layer in tris hydrochloride buffer. The extracted cellulose is removed by low-speed centrifugation.