Insect Tissue Culture: Use of Blastokinetic Stage of Leafhopper Embryo

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Science  19 Jun 1964:
Vol. 144, Issue 3625, pp. 1465-1467
DOI: 10.1126/science.144.3625.1465


To find the proper material for cultivation in vitro of cells of leafhopper vectors of plant viruses, embryonic tissues of the six-spotted aster leaf-hopper (Macrosteles fascifrons) were tested during early developmental stages, during blastokinetic movement, and in late developmental stages. Growing cells were only obtained from the stage of blastokinetic movement. This stage can be determined visually in leafhopper eggs.