Amphibian Cell Culture: Permanent Cell Line from the Bullfrog (Rana catesbeiana)

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Science  26 Jun 1964:
Vol. 144, Issue 3626, pp. 1578-1580
DOI: 10.1126/science.144.3626.1578


A line of fibroblast cells has been established from tongue tissue of the bullfrog (Rana catesbeiana). The cells are near-triploid and were subcultured 57 times during the 22/3 years of their existence. Some of their characteristics are described.