Ultraviolet Sensitivity of Escherichia coli Containing Heat-Inducible λ Prophages

Science  10 Jul 1964:
Vol. 145, Issue 3628, pp. 175-176
DOI: 10.1126/science.145.3628.175


The c1-t mutants of bacteriophage λ can form prophage at 36°C but cause lysis of sensitive bacteria at temperatures above 42°C. Growth of cultures at 42° to 46°C induces prophage replication and lysis in Escherichia coli K12 (λ c1-t); lysogenic strains containing wild-type prophage are not induced to lyse at these temperatures. Heat induction is prevented by chloramphenicol. Strains containing heatinducible prophage are much more sensitive to killing by ultraviolet light than is K12 (λ+).