Histochemical Demonstration of Uptake of Exogenous Norepinephrine by Adrenergic Fibers in vitro

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Science  31 Jul 1964:
Vol. 145, Issue 3631, pp. 503-504
DOI: 10.1126/science.145.3631.503


Adrenergic fibers in isolated iris preparations were demonstrated histochemically by means of the formaldehyde condensation technique. In the presence of adenosine triphosphate (ATP) there was a net uptake of norepinephrine added in vitro by the adrenergic fibers producing a striking increase in fluorescence. In the absence of ATP only a slight increase in fluorescence was observed when the tissue was exposed to high concentrations of norepinephrine.