Differential Estimation of Gamma-butyrolactone and Gamma-hydroxybutyric Acid in Rat Blood and Brain

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Science  07 Aug 1964:
Vol. 145, Issue 3632, pp. 583-584
DOI: 10.1126/science.145.3632.583


A sensitive and specific gas chromatographic technique for estimating concentrations of the anesthetic adjuvants, γ-butyrolactone and γ-hydroxybutyrate, in tissues has been developed. These substances do not appear to occur endogenously in either the blood or the brain of common laboratory animals. The onset and duration of anesthesia caused by the administration of either compound is correlated with the concentration of γ-hydroxybutyrate in the brain, rather than with that of the corresponding lactone.