Electron Microscopic and Biochemical Studies of Pyruvate Dehydrogenase Complex of Escherichia coli

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Science  28 Aug 1964:
Vol. 145, Issue 3635, pp. 930-932
DOI: 10.1126/science.145.3635.930


Examination with the electron microscope of the pyruvate dehydrogenase complex and its component enzymes from Escherichia coli indicates that the complex has a polyhedral structure with a diameter of about 300 to 350 angstroms and a height of 200 to 250 angstroms. The reconstituted complex closely resembles the native complex in appearance. A tentative model of this multienzyme complex is proposed on the basis of the correlated biochemical and electron-microscopic data