Enzymatic Dissection of the Mammalian Ovary

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Science  02 Oct 1964:
Vol. 146, Issue 3640, pp. 73-74
DOI: 10.1126/science.146.3640.73


Incubation of the ovaries of rats and mice with the proteolytic enzyme "pronase" caused the step by step dispersal of cellular elements from the tissue. The first subunits to be liberated were the outermost follicles or corpora lutea or both. Next, these subunits were dispersed. It is possible to obtain isolated single follicles in all stages of development: corpora lutea, isolated cell layers from within follicles, suspensions containing free cells, and ova in all phases of maturation.