Endive Plantlets from Freely Suspended Cells and Cell Groups Grown in vitro

See allHide authors and affiliations

Science  02 Oct 1964:
Vol. 146, Issue 3640, pp. 76-77
DOI: 10.1126/science.146.3640.76


Callus tissue derived from mature embryos of the endive, Cichorium endivia Linn. (family Compositae) grows and develops chlorophyll on a completely defined nutrient medium. The tissue breaks up into a thick suspentsion of cells and cell groups in a liquid medium kept in a flask on a shaker. Gradually, many small round masses of tissue, designated here as embryoids, are formed; these become differentiated and organized to form numnerous small plantlets having typical curled and fringed green leaves and roots.