Phytohemagglutinin Elicitation of Specific Anamnestic Immune Response in vitro

Science  09 Oct 1964:
Vol. 146, Issue 3641, pp. 247-248
DOI: 10.1126/science.146.3641.247


Fragments of lymph nodes from rabbits hyperimmunized with human chorionic gonadotropin or bovine plasma albumin were cultivated in organ culture for 1 month. An anamnestic response was elicited in vitro by the sensitizing antigen. Phytohemagglutinin also produced such a response. Throughout cultivation the cultures showed excellent preservation of morphological integrity and of capacity to produce antibody.