Antigen-Binding Activity of 6S Subunits of β2-Macroglobulin Antibody

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Science  16 Oct 1964:
Vol. 146, Issue 3642, pp. 404-405
DOI: 10.1126/science.146.3642.404


Direct evidence is provided for the antigen binding activity of the 6S subunits formed by reduction and alkylation of rabbit β2-macroglobulin antibody. Binding activity of the subunits was clearly demonstrated by radioimmunoelectrophoresis with a preparation of purified rabbit antibody against the p-azobenzenearsonate group, which contained 40 percent of β2M-antibody. The precipitate arc formed between the subunits and sheep antiserum against rabbit macroglobulin was shown to bind radioactive antigen specifically.