Protein Purification by Elution Convection Electrophoresis

Science  16 Oct 1964:
Vol. 146, Issue 3642, pp. 406-407
DOI: 10.1126/science.146.3642.406


Purified protein components can be recovered from high-resolution gel-electrophoresis patterns by elution-convection electrophoresis. The protein components are all simultaneously eluted from the gel pattern by horizontal electrophoresis and are then simultaneously concentrated by electro-convection to recover the proteins in nearly the original sample concentration. Thirty individual protein fractions can be recovered in 3 hours from a sample containing 200 milligrams of protein.