Chromatography of Ribonuclease Treated Myosin Extracts from Early Embryonic Chick Muscle

Science  16 Oct 1964:
Vol. 146, Issue 3642, pp. 413-414
DOI: 10.1126/science.146.3642.413


Chromatography, on diethylaminoethyl cellulose, of leg muscle extracts from older chick embryos yields three myosin-containing fractions, whereas material from muscle extracts of 11-day embryos cannot be eluted under the same conditions. A major fraction having the adenosine triphosphotase activity characteristic of myosin can be eluted when 11-day-old embryo muscle extracts are given prior treatment with ribonuclease.