Antibody Formation in vitro by Separated Spleen Cells: Inhibition by Actinomycin or Chloramphenicol

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Science  30 Oct 1964:
Vol. 146, Issue 3644, pp. 659-661
DOI: 10.1126/science.146.3644.659


The formation of 19S antibody to poliovirus by rabbit spleen cells in vitro was interrupted by adding actinomycin D (1 to 10 micrograms per milliliter) for 30 minutes or longer. Shorter treatment (5 to 15 minutes) caused partial suppression. Antibody formation was slowly renewed upon removal of the drug. These results suggest that 19S antibody formation is contingent upon DNA-dependent RNA synthesis and that the genetic messenger has an average lifetime of one-half day or less. Treatment with chloramphenicol (20 to 50 micrograms per milliliter) for 3 days caused the cessation of 19S antibody formation.