Polyoma Virus: Production in Bacillus subtilis

See allHide authors and affiliations

Science  06 Nov 1964:
Vol. 146, Issue 3645, pp. 778-779
DOI: 10.1126/science.146.3645.778


Particles of complete polyoma virus are produced in competent Bacillus subtilis incubated with DNA isolated from purified, conventionally grown polyoma virus. The virus grown in B. subtilis is biologically identical to polyoma virus produced by animal cells. Quantitative parameters of the system have been established, and fluctuation tests indicate that viral replication occurs within the infected bacteria.