Culture of Insect Salivary Glands in a Chemically Defined Medium

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Science  20 Nov 1964:
Vol. 146, Issue 3647, pp. 1063
DOI: 10.1126/science.146.3647.1063


A method for culturing the salivary glands of Sciara coprophila in a chemically defined medium has been developed. During a 24-hour period, the polytene chromosomes from six larval stages undergo normal developmental mental changes, including puff formation, puff condensation, and DNA synthesis.