Association of Rapidly Metabolized DNA and RNA

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Science  20 Nov 1964:
Vol. 146, Issue 3647, pp. 1066-1069
DOI: 10.1126/science.146.3647.1066


An association of rapidly metabolized DNA and RNA labeled with P32 has been found in peanut cotyledons by incorporation of either uridine-C14 or thymidine-C14 by peanut cotyledon slices. Chromatography of the extracted nucleic acids on methylated- albumin columns shows that the rapidly metabolized DNA and RNA are contained only in the first half of the DNA peak where RNA and DNA occur in equal amount. One-fourth of the radioactivity is recovered in ribonuc'eotides. Density-gradient centrifugation in cesium chloride indicates that the material containing the P32 has a higher density than most of the DNA.