Cycloheximide: Aspects of Inhibition of Protein Synthesis in Mammalian Cells

See allHide authors and affiliations

Science  11 Dec 1964:
Vol. 146, Issue 3650, pp. 1474-1476
DOI: 10.1126/science.146.3650.1474


Cycloheximide and acetoxy-cycloheximide specifically inhibit protein synthesis in L-cells growing in suspension culture. In extracts of rat liver, the drugs inhibit transfer of amino acid from soluble RNA to polypeptide. Unlike puromycin, these drugs do not accelerate release of nascent polypeptide chains. The drugs have no effect on protein synthesis in extracts of Escherichia coli.