Bone Cells: Biochemical and Biological Studies after Enzymatic Isolation

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Science  11 Dec 1964:
Vol. 146, Issue 3650, pp. 1476-1477
DOI: 10.1126/science.146.3650.1476


Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase, and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.