Mitomycin C: Effect on Ribosomes of Escherichia coli

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Science  18 Dec 1964:
Vol. 146, Issue 3651, pp. 1585-1587
DOI: 10.1126/science.146.3651.1585


Sucrose-gradient analyses of cell-free extracts of Escherichia coliB show that the sedimentation patterns are markedly affected by exposure of the cells to mitomycin C; 50S subunits are progressively degraded in the cells after 30 minutes exposure. The 30S subunits, although affected, are less sensitive to the antibiotic than the 50S subunits. Incorporation of uracil-2-C14 into the ribosomal particles by the treated cells is markedly reduced by exposure.