Constant Volume, Self-Filling Nanoliter Pipette: Construction and Calibration

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Science  05 Feb 1965:
Vol. 147, Issue 3658, pp. 606-608
DOI: 10.1126/science.147.3658.606


Pipettes with volumes ranging from less than one nanoliter to 200 nanoliters can be constructed by means of a simple mechanical system and calibrated by radioisotope and fluorescence techniques. Biological fluids can be transferred with a repeatability of 1 percent by this self-filling pipette of constant volume.