Polysomes from Yeast: Distribution of Messenger RNA and Capacity to Support Protein Synthesis in vitro

Science  05 Feb 1965:
Vol. 147, Issue 3658, pp. 615-617
DOI: 10.1126/science.147.3658.615


Individual fractions of polysomes were isolated from yeast. Pulse labeling experiments in vivo show constant specific activity of messenger RNA in each polysome peak; this suggests a uniform density of ribosomes per unit length of messenger RNA. In the cell-free incorporating system, the amount of peptide per ribosome unit increased with the size of polysome.