Lens Fiber Differentiation and Gamma Crystallins: Immunofluorescent Study of Wolffian Regeneration

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Science  12 Mar 1965:
Vol. 147, Issue 3663, pp. 1299-1301
DOI: 10.1126/science.147.3663.1299


From the adult lens of Triturus viridescens, a fraction of proteins was isolated which corresponds to γ-crystallins of higher vertebrates. Tests by immuno-electrophoresis indicate that the antiserum against this fraction reacts with γ-crystallins, but not with α- or β-crystallins. With this antiserum, an immunofluorescent reagent has been prepared for detection of γ-crystallins from newts. In the normal lens of the adult newt, these crystallins are detected in fiber cells and fiber material, but not in the epithelial cells. During transformation of the iris into the lens after lens removal, the staining reaction is negative in the regenerating tissue up to the time the prospective primary fiber cells begin to elongate. Subsequently, without exception those cells in fiber differentiation indicate a γ-crystallin reaction. When the secondary fiber cells are produced at the equatorial zone of the regenerating lens, they also begin to show a γ-crystallin reaction. Thus, γ-crystallins characterize fiber differentiation.