Synthesis of Block Oligonucleotides

See allHide authors and affiliations

Science  12 Mar 1965:
Vol. 147, Issue 3663, pp. 1310-1311
DOI: 10.1126/science.147.3663.1310


The concentration of sodium chloride strongly influences primed polymerization of nucleoside diphosphates by polynucleotide phosphorylase. High concentrations of NaCl allow the addition of only a few nucleotide residues onto the 3' end of the primer. When pure oligonucleotides are used as primers and when the product is fractionated, pure block oligonucleotides can be obtained.