Serum Protein Electrophoresis in Acrylamide Gel: Patterns from Normal Human Subjects

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Science  19 Mar 1965:
Vol. 147, Issue 3664, pp. 1451-1453
DOI: 10.1126/science.147.3664.1451


Technical improvements in the method for vertical acrylamide gel electrophoresis (Raymond) have resulted in improved resolution and reproducibility. In a survey of approximately 200 normal human serums, ten lines were found to be common in all specimens. Interposed with these constant lines and in addition to known variations in the pattern reflecting haptoglobin and transferrin types, at least one line followed that of albumin and three followed that of transferrin; the presence or absence of these varied from individual to individual. Based on these variations, the electrophoretic patterns of normal humans may be divided into at least 12 discrete groups.