Agrobacterium tumefaciens: Thermal Inactivation of Tumor-Inducing Ability

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Science  26 Mar 1965:
Vol. 147, Issue 3665, pp. 1578-1579
DOI: 10.1126/science.147.3665.1578


Treating cultures of Agrobacterium tumefaciens at 39° to 48°C reduces the infectivity of the bacteria without necessarily affecting viability. Destruction of the capacity to initiate tumor growth follows first-order kinetics from which rate constants for thermal inactivation are derived. From these rates, values for heat of activation of 56.7 kcal mole-1 and entropy of activation of 107 cal mole-1 deg-1 are obtained. A particular protein or nucleoprotein active in the process of infection may be inactivated by the treatment.