Retinoyl Beta-Glucuronic Acid: A Major Metabolite of Vitamin A in Rat Bile

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Science  02 Apr 1965:
Vol. 148, Issue 3666, pp. 86-87
DOI: 10.1126/science.148.3666.86


The major metabolite in rat bile of injected C14-retinoic acid was purified by ion-exchange and silicic acid chromatography; it has the spectrum of methyl retinoate, releases retinoic acid upon basic hydrolysis or by treatment with β-glucuronidase, and contains glucuronic acid. The metabolite was characterized by treatment with diazomethane followed by hexamethyl-disilazane, or with periodate followed by semicarbazide, and the products were chromatographed. The metabolite has been tentatively identified as retinoyl β-glucuronide.