Vaccinia Virus Directed RNA: Its Fate in the Presence of Actinomycin

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Science  02 Apr 1965:
Vol. 148, Issue 3666, pp. 87-90
DOI: 10.1126/science.148.3666.87


RNA similar in base composition to viral DNA is formed in the cytoplasm of HeLa cells infected with vaccinia virus. This RNA is found both in polyribosomes and in a broad 30S to 74S region in a sucrose density gradient. When infected cells are exposed to actinomycin, the amount of this DNA-like RNA in the 30S to 74S region is unchanged. Under the same conditions, 70 percent of the polyribosome-associated DNA-like RNA is degraded to acidsoluble fragments within 2 hours, and protein synthesis is reduced by 90 percent.