Glucose-6-Phosphatase: Reexamination of the RNA-Induced Activity in Mouse Ascites Tumor Cells

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Science  23 Apr 1965:
Vol. 148, Issue 3669, pp. 513-516
DOI: 10.1126/science.148.3669.513


The glucostat method for the measurement of glucose released by glucose-6-phosphatase has been reexamined; it is accurate and sensitive enough to measure glucose-6-phosphatase activity in mouse ascites tumor cells. In seven different experiments, treatment with RNA from liver led to increases in enzyme activity varying between 125 to 200 percent. The activity of induced enzyme is optimum from pH 6 to 6.4.