Enzymatic Synthesis of Tri- and Tetranucleotides of Defined Sequence

See allHide authors and affiliations

Science  30 Apr 1965:
Vol. 148, Issue 3670, pp. 632-634
DOI: 10.1126/science.148.3670.632


Conditions have been found under which polynucleotide phosphorylase adds only a few nucleotide residues to the 3' end of a dinucleotide primer. Pure tri- and tetranucleotides can be isolated from reaction mixtures in high yield.