Mutation of the Blue-Green Alga, Anacystis nidulans

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Science  02 Jul 1965:
Vol. 149, Issue 3679, pp. 70
DOI: 10.1126/science.149.3679.70


Cells of Anacystis nidulans in the logarithmic phase of growth were briefly treated with the mutagen, N-methyl-N'-nitro-N-nitrosoguanidine, and plated under conditions suitable for single-cell growth. Selection of aberrant colonies and examination of the cultural characteristics of these clones suggest that mutants of at least several types are easily derivable in Anacystis.