Polyribosomes from Escherichia coli: Enzymatic Method for Isolation

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Science  06 Aug 1965:
Vol. 149, Issue 3684, pp. 647-649
DOI: 10.1126/science.149.3684.647


Polyribosomes can be rapidly extracted from Escherichia coli by sequential passage of the cells through solutions of a chelating agent and lysozyme in a centrifugal field. Biosynthesis of protein in whole cells and in a cell-free system occurs almost exclusively in the polyribosomes.