Focal Antibody Production by Transferred Spleen Cells in Irradiated Mice

Science  27 Aug 1965:
Vol. 149, Issue 3687, pp. 998-1000
DOI: 10.1126/science.149.3687.998


Lethally irradiated mice were injected with small numbers of normal spleen cells and then immunized with sheep erythrocytes. Antibody activity was found in their spleens in localized areas whose number corresponded to the number of spleen cells injected. When sheep and pig erythrocytes were injected together, antibody against each was found in separate areas. Each area may consist of the progeny of a single precursor cell, restricted to forming a single antibody.