Lability of Host-Cell DNA in Growing Cell Cultures Due to Mycoplasma

Science  03 Sep 1965:
Vol. 149, Issue 3688, pp. 1098-1099
DOI: 10.1126/science.149.3688.1098


In HeLa-cell cultures chronically infected with Mycoplasma, (PPLO) host-cell DNA is unstable as detected by incorporation of H3-or C1 i-thymidine into DNA and subsequent release into the medium as acidsoluble radioactivity. This characteristic can be transmitted to PPLO-free cultures of strain L cells by inoculation with preparations of PPLO from the HeLa cells, although chronically infected cultures of L cells continue to multiply. In addition, virus preparations also may carry PPLO contamination through numerous passages.