Nucleoside Incorporation into Strain L Cells: Inhibition by Pleuropneumonia-Like Organisms

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Science  03 Sep 1965:
Vol. 149, Issue 3688, pp. 1100-1101
DOI: 10.1126/science.149.3688.1100


Contamination of Strain L cell cultures by pleuropneumonia-like organismtis (PPLO) resulted in a complete inhibition of the incorporation of tritiated thymnidine and uridine. Contanminated cultures were characterized by a concentration of PPLO along the margins of the cells and in the intercellular-spaces of the cultures. Autoracliograins of PPLO-contaminated cultures were characterized by exposed silver grains over the margins of the cells. Kanamnycin was efjective in the elimination of PPLO and the restoration of nucleoside incorporation.