Anesthesia of Artemia Larvae: Method for Quantitative Study

Science  10 Sep 1965:
Vol. 149, Issue 3689, pp. 1255-1258
DOI: 10.1126/science.149.3689.1255


Potency of anesthetics is quantitatively measured with laboratory-hatched larvae of the brine shrimp Artemia salina. Statistical fluctuations are minimized in that 100,000 animals are used to determine a single median anesthetic dose value. The technique was developed to study molecular mechanisms of general anesthesia.