Mercurial-Induced Transformation of Myosin Prevented by Adenosine Triphosphate and Pyrophosphate

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Science  17 Sep 1965:
Vol. 149, Issue 3690, pp. 1374-1375
DOI: 10.1126/science.149.3690.1374


Adenosine triphosphate and pyrophosphate prevent the loss of Ca÷÷-activated adenosine triphosphatase activity caused by high concentrations of mercurial sulfhydryl reagent. They concomitantly prevent the transformation of myosin into faster-sedimenting products. This is adduced as support for the hypothesis that the strategic sulfhydryl group is not binding adenosine triphosphate at the active site, but is initiating a conformational change upon its reaction with the mercurial reagent.