Prostaglandin: Release from the Rat Phrenic Nerve-Diaphragm Preparation

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Science  17 Sep 1965:
Vol. 149, Issue 3690, pp. 1390-1391
DOI: 10.1126/science.149.3690.1390


Release of a substance that stimulates smooth muscle was detected from a phrenic nerve-diaphragm preparation from the rat upon direct and indirect stimulation. On thin-layer chromatography most of the active material behaved as a mixture of prostaglandins. The effect of electrical stimulation was mimicked by catecholamines but not by acetylcholine or eserine. The effect of nerve stimulation was not antagonized by d-tubocurarine.