Constitution, Viability, and Lactate Dehydrogenase in Stationary-Phase L-Cell Suspension Cultures

See allHide authors and affiliations

Science  15 Oct 1965:
Vol. 150, Issue 3694, pp. 350-353
DOI: 10.1126/science.150.3694.350


Starved suspension cultures of L cells exhibit a plateau phase of short duration followed by loss of key cellular constituents and rapidly decreasing viability. In contrast, regularly fed,. undiluted cultures remain stationary at a high cell density for prolonged periods without structural alterations or loss of viability. The L cells contain a single lactate dehydrogenase isozyme with an electrophoretic mobility similar to that of lactate-dehydrogenase-5. High-density stationary cultures show a tenfold increase of the specific activity of this enzyme and a recurrent biphasic pattern of carbohydrate utilization with, first, production and, later, consumption of lactate.