Ribosomes: Analysis by Cesium Sulfate Gradient Centrifugation

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Science  29 Oct 1965:
Vol. 150, Issue 3696, pp. 610-612
DOI: 10.1126/science.150.3696.610


Ribosomes from the cytoplasm of a mammalian tissue, HeLa cells, were partially purified and then concentrated into a zone by prolonged centrifugation in Cs2SO4 solution (density, 1.43 grams per milliliter). Ribosomal preparations treated with bentonite prior to density-gradient centrifugation showed single peaks after centrifugation. Material recovered from the peaks represented aggregates of the original 74S particle.