Sperm Capacitation by Uterine Fluid or Beta-Amylase in vitro

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Science  29 Oct 1965:
Vol. 150, Issue 3696, pp. 618-619
DOI: 10.1126/science.150.3696.618


Rabbit sperm developed the capacity to fertilize ova when incubated in utero or in vitro in fluids from estrous uteri. Incubation of sperm with beta-amylase in phosphate-buffered Locke's solution also resulted in capacitation. The data suggest that sperm capacitation involves enzymatic alteration of carbo-hydrate-containing seminal macromolecules which coat sperm and inhibit fertilization.