Proteins and Disulfide Groups in the Aggregation of Dissociated Cells of Sea Sponges

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Science  14 Jan 1966:
Vol. 151, Issue 3707, pp. 203-205
DOI: 10.1126/science.151.3707.203


A sponge extract that produced specific aggregation of dissociated cells was treated with various enzyme preparations to determine which enzymes would destroy its aggregating properties. The results indicate that proteins play a key role in the aggregating effect of the extract on dissociated, glutaraldehyde-fixed sponge cells. Further studies confirm the necessity of calcium for the aggregation and indicate the necessity of intact disulfide groups.