Medium for Selective Isolation of Cryptococcus neoformans

+ See all authors and affiliations

Science  14 Jan 1966:
Vol. 151, Issue 3707, pp. 208-209
DOI: 10.1126/science.151.3707.208


A medium has been developed that permits the selective recovery of Cryptococcus neoformans from heavily contaminated materials. It employs creatinine as a nitrogen source, diphenyl (C6H5C6H5) and chloramphenicol as mold and bacterial inhibitors, and Guizotia abyssinica seed extract as a specific color marker. The medium has proved to be effective in the direct isolation of Cryptococcus neoformans from pigeon nests and from the air.