Trehalase from Dictyostelium discoideum: Purification and Properties

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Science  28 Jan 1966:
Vol. 151, Issue 3709, pp. 454-456
DOI: 10.1126/science.151.3709.454


The hydrolytic enzyme, trehalase, was isolated and purified approximately 90-fold from the cellular slime mold Dictyostelium discoideum. The purified trehalase has an optimal temperature of 45°C and shows maximum activity at pH 5.5 in citrate buffer. Its Michaelis constant is 1.2x 10-3M.