Synchronization of Mammalian Cells with Tritiated Thymidine

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Science  11 Feb 1966:
Vol. 151, Issue 3711, pp. 691-694
DOI: 10.1126/science.151.3711.691


Short exposures of mammalian cells to tritiated thymidine of high specific activity destroys the proliferative capacity of mammalian cells. Since the killing is limited to cells that have synthesized DNA in the presence of the labeled compound, an exposure duration of less than one generation can yield a synchronized population.