Sedimentation of an Initially Skewed Boundary

Science  15 Apr 1966:
Vol. 152, Issue 3720, pp. 359-361
DOI: 10.1126/science.152.3720.359


An asymmetric initial boundary is often formed when a synthetic boundary cell is used in the analytical ultracentrifuge. Model calculations show that, when such a boundary diffuses, the peak of the corresponding gradient pattern moves. The movement of the peak resulting from diffusion, when superimposed on the sedimentation of the boundary, may produce errors in the measurement of the sedimentation coefficient.