Replication of the RNA of Bacteriophage f2

See allHide authors and affiliations

Science  15 Apr 1966:
Vol. 152, Issue 3720, pp. 372-377
DOI: 10.1126/science.152.3720.372


Events occurring after infection of bacteria with wild-type and a temperature-sensitive mutant phage indicate that there are two enzymatic activities necessary to replicate phage RNA. One converts single strands into double strands, while the other uses double strands to svnthesize viral RNA. The mutant is deficient in the first activity, probably because the mutation is in the gene specifying the requisite enzyme. On the basis of these and other results, a model is presented for the replication of phage RNA.