Reports

Zonal Ultracentrifuge for the Separation of Ribosomal Subunits

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Science  15 Apr 1966:
Vol. 152, Issue 3720, pp. 378
DOI: 10.1126/science.152.3720.378

Abstract

Ribosomal subunits were prepared by means of the Anderson zonal ultracentrifuge. With this technique it is possible to separate more than 300 milligrams of subunits with complete resolution in a single run, as compared with about 2 milligrams with conventional rotors. Superior resolution is achieved in the zonal ultracentrifuge, affording possibilities for the detection and preparation of minor ribosomal components.

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