Zonal Ultracentrifuge for the Separation of Ribosomal Subunits

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Science  15 Apr 1966:
Vol. 152, Issue 3720, pp. 378
DOI: 10.1126/science.152.3720.378


Ribosomal subunits were prepared by means of the Anderson zonal ultracentrifuge. With this technique it is possible to separate more than 300 milligrams of subunits with complete resolution in a single run, as compared with about 2 milligrams with conventional rotors. Superior resolution is achieved in the zonal ultracentrifuge, affording possibilities for the detection and preparation of minor ribosomal components.