Biosynthesis and Composition of Histones in Novikoff Hepatoma Nuclei and Nucleoli

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Science  22 Apr 1966:
Vol. 152, Issue 3721, pp. 521-523
DOI: 10.1126/science.152.3721.521


Histones were prepared from isolated nuclei and nucleoli of the Novikoff ascitic hepatoma at several time points after the injection of L-lysine uniformly labeled with C14 into tumor-bearing rats. Amino acid analysis and starch-gel electrophoresis failed to reveal any differences between the nuclear and nucleolar histones, although both fractions were more acidic in composition than calf thymus histones. However, the nucleolar histones were a metabolically distinct fraction, and their rate of synthesis was approximately twice that of the total nuclear histones.